Motivul este că preceptele fundamentale sunt în concordanță cu Vedele și alte scripturi ortodoxe ale hindușii sanatane Dharma. The employed method allowed identification and determination of six compounds and they are listed in Table 1 , together with the analytical performances of the method.
For the analytical evaluation of polyphenolic compounds, a small quantity of the extract was used for chromatographic analysis. The elution was performed at room temperature, on a distance of 8 cm and using normal chromatographic twin chambers Camagwhich were pre-saturated with the mobile phase for 30 minutes.
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The developed plates were heated at °C for about 3 minutes and then were immersed in Natural Products solution NP - 1g diphenylborinic acid aminoethylesterdissolved in mL ethyl acetate and then dried in cold air followed by another immersion in polyethylene glycol PEG— 10 g polyethylene glycol dissolved in mL dichloromethane. Compound detection was obtained after immersion in the two solutions mentioned above, using visible and UV light at and nm.
A HPLC-DAD method was optimized in order to separate and quantitatively determinate the compounds of interest, parashar light an Agilent HPLC system Waldbronn, Germany equipped with an on-line vacuum degasser, quaternary pump, temperature controlled sample tray, automatic injector, a column thermostat compartment and a DAD detector.
The chromatographic separations were run on a Zorbax C18 column mm x 4. The injection volume was 5 μL 0.
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Several preliminary tests were run by varying the experimental conditions gradient, injection volume, wavelength detection, etc. The optimum method consisted of a gradient elution using solvent A, 5 mM ammonium acetate pH 5.
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Standards of chlorogenic acid, p-coumaric acid, cafeic acid, ferulic acid, rutin, hyperoside, isoquercitrin, quercetin, kaempferol, luteolin, and jatrorrhizine an alkaloid were employed, all of analytical grade purity for different commercial available sources.
The UV-Vis detection of the compounds was carried out parashar light the DAD detector that measured the entire spectrum in — nm region every 1 s; the chromatograms were monitored at parashar light,, and nm.
Identification of the compounds was achieved by the chromatographic retention time with a 0. The chromatograms were exported and the graphs were developed in Excel. Acid hydrolysis of the extract was carried on in 2 M hydrochloric acid at 80°C for one hour, followed by filtration of a slight precipitate that was formed.
The extract turned into deep green after hydrolysis, specific to decomposition and oxidation of chlorogenic acid and its derivates.
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Antioxidant and pro-oxidant activity evaluation The antioxidant activity of the hydro alcoholic extract of G. The parashar light reactivity of the extract was evaluated using a previously developed method that is described in detail elsewhere [ 15 ]. Briefly, the extract is treated with a catalytic amount of laccase that generates radicals from the components of the extract which are responsible of oxidation of the ferrous oxy hemoglobin oxyHb into the oxidized form metHb.
The kinetic profile and the rate of the parashar light oxidation is a marker for the reactivity of the generated radicals. EPR spectroscopy measurements The protocol for the Electron Paramagnetic Resonance EPR -based investigation is described fully described elsewhere [ 16 ], with slight modifications. Briefly, in a 1.
The spectra were recoded using a Bruker EMX Spectrometer with continuous wave at X band ~9 GHz at room temperature with following parameters: microwave power 9 mW, modulation amplitude 1 G, center field G, and sweep field of 50 G.
Handling was performed quietly and gently. The standard diet and tap water were ad libitum.
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Study design The animals were randomly assigned in 4 groups of 6 animals each, as follows: C Control—unstressed animalsS animals exposed to restraint stress and darkness, in the same time parashar light, SG1 animals exposed to stress and treated with G. The hydroalcoholic extract was administrated via oral gavage, every day at the same periods of time 10 a. We aimed to investigate if the G. Blood and tissue sampling At the end of the experiment 7 days the animals were sacrificed by decapitation under anesthesia with ketamin- xylazine cocktail 60 mg ketamine and 7.
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Blood was collected and centrifuged in order to obtain blood serum, which was frozen at—70° C, until analysis. Afterwards, they were embedded in paraffin and sectioned at 5 μm with a microtome Reichert, Austria. The sections were histo-processed and colored using hematoxylin-eosine staining protocol.
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Biochemical parameters Blood serum was used for the determination of total proteins TPcreatinine concentrations Creacatalase activity CATthe concentration of thiobarbituric acid reactive substances TBARSaspartate aminotransferase activity ASTalanine aminotransferase activity ALTcorticosterone and epinephrine levels, using a semi-automatic biochemistry analyser Evolution that executes photometric measurements parashar light elaborates them according to the corresponding programs, and a UV PC spectrophotometer.
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CAT activity was determined by a kinetic method, using H2O2 as substrate, according to [ 19 ]. The decreasing absorbance of H2O2 was measured at nm.
SOD activity was measured based on a method described by [ 20 ]. TBARS levels were estimated according to a modified method of [ 21 ]. The reaction mixture was determined spectrophotometrically at nm. Serum hormone levels were determined using ELISA commercial kits Biomaxima, Polandwhich included controls and standards for determination of the analyte.
Figures Abstract Galium verum is a well-known medicinal plant which is used in various pathologies. Considering the chemical findings, the potential beneficial effects of the G. Thus, the biochemical-modulatory and antioxidant roles of two doses of G. The animals were divided in groups [control, S, SG1 exposed to 25 mg G. The G.
Statistical methods All data are reported as the mean ± SEM. The Gaussian distribution was checked by the Shapiro-Wilk normality test. Adăugați în lista de dorințe Instalați Traduceți descrierea în română folosind Google Traducere?
Lord Swaminarayan wrote the Shikshapatri towards the end of his lifetime over one hundred and seventy five years ago. However, the underlying principles apply to mankind even today and will undoubtedly apply to all for eternity. The reason being is that the fundamental precepts are consistent with the Vedas and other orthodox scriptures of Hindu Sanatan Dharma.
The Lord wrote the Shikshapatri for the benefit of mankind - so that all could read and understand thebasic fundamental commandments applying to all Hindus.
The contents of the Shikshapatri have been sieved from numerous Dharma Shastras treatise upon Dharma from parashar light likes of Manusmruti, Yagnavalkya Smruti, Parashar Smruti etc. It has three languages-English,Gujarati and Sanskrit.